The physicochemical interactions between macromolecular components create a cellular machinery of great complexity. Whereas the core molecular repertoire has been identified and biochemically characterized, we understand very little about how the cell functions as a whole. To visualize and understand the orchestration of macromolecular machines we use an arsenal of biophysical technologies such as advanced microscopy techniques, high-resolution structural methods, molecular modeling, but primarily cryo-electron tomography combined with machine-learning techniques. These technologies we use to understand adhesion mechanisms in both eukaryotes and bacteria. To enable adhesion the cell develop intricate structures with complex architectures. Dysfunction of those have adverse effect in maintaining cellular homeostasis and contribute to development of disease like cancer and drug resistance in bacteria.
Figure: Imaging across scales with different biophysical methods. From the molecular, cellular, tissue and organism level a multitude of informations is collected in order to address various questions essential to life.
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